Human evolution Essay Example for Free

Human evolution Essay Australopithecus apheresis separated humans directly from other primates because they walked upright. There is a huge evolutionary connection between brain size and bipedalism, Australopithecus and our other ancestors brain size was increased because of a change in their diets. Before, our relatives were strict herbivores until they became bipedal and ate meat because it was more available. Which meant that our early ancestors had to run to catch there food, thus increased their brain size because their brains were used eating vegetables. Natural selection has played an important role human evolution. Mutations and natural selections such as change in pigmentation and bacteria inside our body have to help us survive. Random mutations occur in our DNA that can befit, harm or do nothing. Natural selection happens in evolution to help our ancestors to adapt their surroundings. For instance, humans lost their body hair to free themselves of external parasites that infest fur. Over the time of evolution our ancestors body lost what it didnt need and kept what it need. As our ancestors started to move bipedal their body naturally selected to devolve there opposable toe. This was an advantage because our ancestors could run faster and have better alignment with their skeleton. Other natural selection happened over the time of evolution that devolved bodys and evolved bodies. The discovery of controlling of fire was the rising point of human evolution and culture, fires helped humans cook food and gives them comfort. According to James, Steven a Professional form the university of Chicago, the first clam for the earliest evidence of a controlled fire was by a member of a homo that dates back from 1. 7 million years ago. There is justified evidence that early humans behaviour and diet changed when they learned to control fire. The discovery of fire by early human let them say active in night-time hours. A professor from Harvard university claims as early human used fire for cooking food it also expanded and developed their brains because they were allowing more complex carbohydrates in to their bodies. Eating cooked food let our ancestors have more time for them because they were no longer trying to chew on a hard plant allay trying to gain calories from it. When our ancestors starts to cook their food their teeth, jaws, digestive traps and sexual consistencys started to shrink, all of these changes stated happening about 1. 8 million years ago, additionally this is more evident on the Homo erectus. As food became less of a concern to early human, weapons became more sophisticated. When early humans starts using tool evolution helped advance the development and size of their brains. Evolution gave our ancestors an advantage over chimpanzee, muscles that that chimpanzees lack. Humans have thicker fingers with wider heads, making it easier for humans to hold on to objects and throw them more accurately compared to chimpanzees hands. Although, hominid the australopithecine had small sized brains compared to the chimpanzee, they tried very often to cut through tough animal skin to take the meat of the bones, but this makes sense because the australopithecine was still a tree climbing animal. The great advance in stone tools happened around the time of the Homo erectus. The Homo erectus was the first of our ancestors to carry tools around, instead of dropping them after using them, like the australopithecines. Plus, the Homo erectus brain had grown over the time of evolution to three times the size on contrast to the australopithecines. As tools use evolved, communication and language was generated. As our ancestors more and more tools, their hands were occupied while they were hunting and couldnt use gesture so they evolved communication. In order for the early humans to survive, they need to co-operate. For instance, the group was sleeping and on human was chosen to stay awake and wants to wake the group up because a woolly mammoth was rear the group, that individual would need to explain what he wants the group to comprehend. The theory of Evolution is a justified and straightforward; it has been extensively studied and supported by all modern science. Creationism in my opinion on the other hand is completely a bunch of lies, faith does not give you answers, and it only asks you to stop asking question. There is no possible way to test creationism, there for believers accept it as the truth. DNA proves that all cells on earth are someway genetically related, for instance humans are 99. 8 the same genetic code as chimpanzees. Evolution happened over millions and millions of year. Creationism states that someone was told to searching out our planet for 8. 7 million place them aboard to Noahs ark. In my opinion I believe evolution didnt over night or the human race was created in one day by a supernatural being because it has been proving with our technology and our discoverys of fossil records that evolution happen because of natural selections. Creationism is for people that dont care about anthropology, sociology and phycology. People that believe in evolution usually are people that dont want go to church and believe in god. Religion celebrates death while evolution celebrates life.

Structural Interconversion of Holin Transmembrane Domain I

Structural Interconversion of Holin Transmembrane Domain I Structural Interconversion of Holin Transmembrane Domain I is Dictated by a Single Proline: A FRET-based Analysis and its Functional Importance in Pore Formation. Muralikrishna Lella,†  Soumya Kamilla,†¡ Vikas Jain†¡,* and Radhakrishnan Mahalakshmi† ,* KEYWORDS (Word Style â€Å"BG_Keywords†). If you are submitting your paper to a journal that requires keywords, provide significant keywords to aid the reader in literature retrieval. ABSTRACT: Mycobacterial cell lysis during the lytic D29 bacteriophage infection is timed by perfect orchestration amongst/between components of the holin-endolysin cassette. In Gram-negative bacteria, progressively accumulating holin in the inner membrane, retained in its inactive form by anti-holin, is triggered into active hole formation, resulting in the canonical host cell lysis. However, the molecular mechanism of regulation and physical basis of pore formation in the mycobacterial inner membrane by D29 holin, particularly in the nonexistence of an anti-holin, is poorly understood. In this study, we report, for the first time, the use of fluorescence resonance transfer measurements to demonstrate that the first transmembrane domain (TM1) of D29 holin undergoes a helix ↔ ÃŽ ²-hairpin conformational interconversion. We validate that this structural malleability is mediated by a centrally positioned proline, and is abolished in the conformationally rigid substitution mutants containing Ala, DPro, or Aib. Using electrophysiology measurements coupled with calorimetric vesicle assays, we demonstrate that due to the conformational switch, native TM1 exhibits sluggish self-association in membrana, while its rigid variants show accelerated lipid bilayer disruption. The biological implications of D29 holin structural alteration is presented as a holin self-regulatory mechanism and its implications are discussed in the context of data-driven peptide-based therapeutics. The fatal host cell lysis step during bacteriophage infection is one of the most precisely programmed events, coordinated by the mechanical membrane disruption by a hole-forming membrane protein termed holin and the peptidoglycan-degrading enzyme endolysin.1 In the canonical holin-endolysin cassette, holin accumulates in the bacterial inner membrane and is retained in the inactive form until membrane depolarization drives holin assembly into holes that are large enough for endolysin release.1b,2 It is believed that ion leak through pinholes formed in holin-enriched lipid rafts would result in local membrane depolarization, which would exponentially propagate throughout the bacterial inner membrane and result in the formation of >300 nm diameter holes.2g,3 Historically, genetics of the coliphages T4, ÃŽ », and 21 have been extensively investigated, and therefore our current understanding of holin function and regulation is largely derived from the lysis effector S105 (or S2168) holin and the antiholin S107 (or S2171).2d,2g,4 Based on more recent functional characterization of members from eight holin superfamilies comprising several bacteriophages,5 we now know that the number of transmembrane ÃŽ ±-helical segments (TMSs) can vary from 1-4; of this, the 3-TMS is widely prevalent.1b,6 Despite conceptual and experimental advances in our understanding of holin function, very few studies have translated these findings to the mechanism of holin regulation in mycobacteriophages (Mφ). Currently, >4000 documented Mφ species exist, of which >600 have been sequenced.7 Of particular interest is the lytic Mφ D29, which is the predator for Mycobacterium tuberculosis, among other mycobacteria. Mφ D29 possesses a putative holin sequence coded by the gp11 gene, and is predicted to possess two transmembrane segments, typically observed in class II holins.8 However, an antiholin sequence is conspicuously missing in the Mφ D29 genome, raising concerns on how this phage achieves holin regulation. A previous finding from our laboratory demonstrated that the first transmembrane domain (TM1) of D29 Mφ holin could undergo a conformational switch from a helical form to an extended structure, and a centrally located Pro-Gly segment was important for such interconversion.9 This opened further questions on the biophysical nature of such a conformational conversion, the functional implications during holin assembly and whether such interconversion did indeed possess any regulational role within the mycobacterial cell. In this study, we demonstrate that the D29 Mφ TM1 undergoes a helix ÃŽ ²-hairpin conversion that is abolished in Pro -> Ala/DPro/Aib mutation. We also show that proline internally regulates assembly of TM1 in the membrane, and could potentially function as the ‘missing’ antiholin in D29 Mφ. RESULTS AND DISCUSSION ASD ASD METHODS Peptide synthesis and labeling with fluorescent probes. All peptides were synthesized using Fmoc chemistry on a Rink Amide AM resin with a 0.63 mmol/g loading capacity, using DMF as the medium. Deprotection of Fmoc was achieved using 20% piperidine and the progress of the reaction was monitored using Kaiser test and mass spectrometry.9-10 Final peptide was generated using the cleavage cocktail (TFA : water : phenol : ethanedithiol : thioanisole in the ratio 85:5:5:2.5:2.5), followed by cold ether precipitation, and verified by mass spectrometry. On-resin labeling of the fluorophore (Alexa Fluor ® 350 or dansyl chloride) at the N-terminal residue was achieved using HOBt or DIPEA in DMF. All labeling reactions were carried out at least twice and confirmed by mass spectrometry. Labeling efficiency was calculated using labeled peptide absorbance at fluorophore ÃŽ »max ­ and unlabeled : labeled peptide ratios for all reactions were maintained at ~1.0:0.5. Details are in the electronic supplementary information (ESI). Peptide folding and circular dichroism experiments. Desired quantity of peptide in the powder form was dissolved in 100 mM LDAO (lauryldimethylamine oxide) or 100 mM DPC (n-dodeyclphosphocholine) micelles prepared in 50 mM sodium phosphate pH 7.2, and were subjected to repeated cycles of heating and vortexing to promote peptide folding.9 All biophysical experiments were carried out using 0.022-0.024 mM samples, unless otherwise specified. Quantification was achieved using a molar extinction coefficient of 8408 M-1 cm-1 at 280 nm.CD spectra were acquired in various micellar conditions at 25  °C, using a 1 mm path length quartz cuvette at scan speeds of 100 nm/min. Data were integrated over three acquisitions and converted to molar ellipticity values using reported methods.9,11 Thermal denaturation and recovery measurements were carried out between 5-95  °C and 95-5  °C, respectively at a ramp rate of 1  °C/min. Details are provided in the ESI. Fluorescence and anisotropy measurements. Steady state Fà ¶rster Resonance Energy Transfer (FRET) measurements were carried out using Trp excitation at 280 nm ( ±2 nm slit width) and emission spectra were recorded between 295-550 nm ( ±3 nm slit width). Inter- and intra-molecular FRET was demarcated by titrating unlabeled peptide into labeled peptide samples to achieve stepwise dilutions and final unlabeled: labeled ratios of 1:1, 1:0.8, 1:0.6, 1:0.4, 1:0.2, 1:0. Data were normalized against Trp emission intensities and acceptor intensity at ÃŽ »max ­ were plotted (Alexa Fluor ® 350 ÃŽ »max = 442 nm and dansyl chloride ÃŽ »max = 500 nm). Anisotropy values were acquired using ÃŽ »ex-max = 442 nm and ÃŽ »em = 345 nm for both the labeled and unlabeled peptides. Lifetime measurements were carried out using time correlated single photon counting. Trp excitation was achieved at 292 nm using a pulsed LED and fluorescence decays were monitored at the ÃŽ »em-max for the respective samples (345 nm in LDAO; 347 nm in DPC; 355 nm in buffer). All data were fitted to a triple exponential decay to derive lifetimes (Ï„i) and their respective amplitudes (ÃŽ ±i). The average lifetime was given as = ÃŽ £Ãâ€ži.ÃŽ ±i.12Details are provided in the ESI. Pore formation measurement using planar lipid bilayers. Black lipid membranes were generated using DiPhPC (diphytanoyl phosphatidylcholine) on a planar lipid bilayer workstation in which the membrane bilayer was painted across a 150 ÃŽ ¼m aperture generated in the septum of a Delrin cup. A constant 10 mV voltage was applied in both cis and trans sides of the chamber, pre-filled with mM sodium phosphate pH 7.2 containing 0.5 M KCl. 0.022-0.024 mM peptide was added to the cis chamber and electric current was recorded using a 50 Hz filter, sampling frequency of 10 kHz, and digitized. Opening and closing event frequency was calculated throughout the recording and converted to conductance using the formula: [observed current in pA] / 10 mV = conductance in nS. Details are described in the ESI. ASSOCIATED CONTENT (Word Style â€Å"TE_Supporting_Information†). Supporting Information. A brief statement in nonsentence format listing the contents of material supplied as Supporting Information should be included, ending with â€Å"This material is available free of charge via the Internet at http://pubs.acs.org.† For instructions on what should be included in the Supporting Information as well as how to prepare this material for publication, refer to the journal’s Instructions for Authors. ABBREVIATIONS CCR2, CC chemokine receptor 2; CCL2, CC chemokine ligand 2; CCR5, CC chemokine receptor 5; TLC, thin layer chromatography. REFERENCES (Word Style TF_References_Section). References are placed at the end of the manuscript. Authors are responsible for the accuracy and completeness of all references. Examples of the recommended formats for the various reference types can be found at http://pubs.acs.org/page/4authors/index.html. Detailed information on reference style can be found in The ACS Style Guide, available from Oxford Press. (1) (a) Smith, D. L.; Young, R. J. Bacteriol. 1998, 180, 4199-4211; (b) Young, R. Current Opinion in Microbiology 2013, 16, 790-797; (c) Young, R. Microbiological Reviews 1992, 56, 430-481. (2) (a) Young, R.; Wang, I.; Roof, W. D. Trends Microbiol. 2000, 8, 120-128; (b) Ramanculov, E.; Young, R. Gene 2001, 265, 25-36; (c) Wang, I. N.; Deaton, J.; Young, R. J. Bacteriol. 2003, 185, 779-787; (d) Park, T.; Struck, D. K.; Deaton, J. F.; Young, R. Proc. Natl. Acad. Sci. U. S. A. 2006, 103, 19713-19718; (e) Savva, C. G.; Dewey, J. S.; Deaton, J.; White, R. L.; Struck, D. K.; Holzenburg, A.; Young, R. Mol. Microbiol. 2008, 69, 784-793; (f) Pang, T.; Savva, C. G.; Fleming, K. G.; Struck, D. K.; Young, R. Proc. Natl. Acad. Sci. U. S. A. 2009, 106, 18966-18971; (g) White, R.; Chiba, S.; Pang, T.; Dewey, J. S.; Savva, C. G.; Holzenburg, A.; Pogliano, K.; Young, R. Proc. Natl. Acad. Sci. U. S. A. 2011, 108, 798-803; (h) Young, R. Journal of Microbiology 2014, 52, 243-258. (3) (a) Dewey, J. S.; Savva, C. G.; White, R. L.; Vitha, S.; Holzenburg, A.; Young, R. Proc. Natl. Acad. Sci. U. S. A. 2010, 107, 2219-2223; (b) Pang, T.; Fleming, T. C.; Pogliano, K.; Young, R. Proc. Natl. Acad. Sci. U. S. A. 2013, 110, E2054-2063; (c) Savva, C. G.; Dewey, J. S.; Moussa, S. H.; To, K. H.; Holzenburg, A.; Young, R. Mol. Microbiol. 2014, 91, 57-65. (4) Blasi, U.; Nam, K.; Hartz, D.; Gold, L.; Young, R. EMBO Journal 1989, 8, 3501-3510. (5) Reddy, B. L.; Saier, M. H., Jr. Biochim. Biophys. Acta 2013, 1828, 2654-2671. (6) Wang, I. N.; Smith, D. L.; Young, R. Annu. Rev. Microbiol. 2000, 54, 799-825. (7) http://phagesdb.org/. (8) (a) Catalao, M. J.; Gil, F.; Moniz-Pereira, J.; Pimentel, M. J. Bacteriol. 2011, 193, 2793-2803; (b) Hatfull, G. F.; Jacobs-Sera, D.; Lawrence, J. G.; Pope, W. H.; Russell, D. A.; Ko, C. C.; Weber, R. J.; Patel, M. C.; Germane, K. L.; Edgar, R. H.; Hoyte, N. N.; Bowman, C. A.; Tantoco, A. T.; Paladin, E. C.; Myers, M. S.; Smith, A. L.; Grace, M. S.; Pham, T. T.; OBrien, M. B.; Vogelsberger, A. M.; Hryckowian, A. J.; Wynalek, J. L.; Donis-Keller, H.; Bogel, M. W.; Peebles, C. L.; Cresawn, S. G.; Hendrix, R. W. J. Mol. Biol. 2010, 397, 119-143; (c) Payne, K.; Sun, Q.; Sacchettini, J.; Hatfull, G. F. Mol. Microbiol. 2009, 73, 367-381. (9) Lella, M.; Mahalakshmi, R. Chem. Commun. 2013, 49, 9594-9596. (10) Makwana, K. M.; Raghothama, S.; Mahalakshmi, R. Phys Chem Chem Phys 2013, 15, 15321-15324. (11) Maurya, S. R.; Chaturvedi, D.; Mahalakshmi, R. Sci. Rep. 2013, 3, 1989. (12) Maurya, S. R.; Mahalakshmi, R. J. Biol. Chem. 2013, 288, 25584-25592.

Comparing Life Without Meaning in Eliot’s The Waste Land and Welles’ Ci

Life Without Meaning in T. S. Eliot’s The Waste Land and Orson Welles’ Citizen Kane It is the foundation of modern civilization that knowledge is better than ignorance, understanding more valuable than confusion, and wisdom more desirable than foolishness. Consequently, people feel that they should be able to understand the meaning of life and, in doing so, know that their lives are not in vain. They want life to be a coherent whole infused with meaning, so that they can know and understand what life is and where they fit in, thereby attaining wisdom. Life, then, is in essence a struggle to find a meaningful framework for the experiences and feelings we have collected. Since art reflects the human state, and we have grown more conscious of this struggle, our art has come to reflect this problem of making a coherent, meaningful whole out of the assorted fragments of life we have collected. Though this theme of collecting is visible in all media of modern art, it is especially noticeable in literary and film art, in particular in T. S. Eliot’s The Waste Land and Orson Welles’s Citizen Kane. These two pieces analyze collecting on two levels: first because they focus on collecting and second because they are collections. However, the division between these levels is somewhat superficial, as both Eliot and Welles blur the lines between the two parallels, making the audience more acutely aware that this art is a picture of all human life. On both levels the artists draw parallels between the works and the audiences’ lives by examining the content, style, and meaning of the collection; that is, what is collected, how it is collected, and what it says, concluding that life is not a coherent whole, but rather â€Å"a heap of broken ima... ...to be. Collecting points out that in the very act of observing the art, we are collecting, as the persona and Kane are, in an attempt to make a coherent and meaningful whole out of our lives, and that we will fail as surely as they do. NOTES 1 T.S. Eliot, â€Å"The Waste Land,† in The Waste Land and Other Poems (New York: Harcourt Brace, 1962). 2 The original title of The Waste Land was a quote from Dickens's novel Our Mutual Friend, "He do the Police in different voices." 3 When I say that the poem lacks a coherent meaning, I mean that it has no more coherent meaning that life itself does. 4 "I am not Russian, I am out of Lithuania, a real German." 5 It is interesting to note the two meanings that this phrase can have. It could mean that the fragments are anchored in his literary tradition, or it could mean that it is set up as a bulwark to prevent his ruin.

Black Americans Struggle in Baldwins Stranger in the Village :: Stranger Village Baldwin Essays

Black Americans' Struggle in Baldwin's Stranger in the Village In ?Stranger in the Village? (1955), James Baldwin, a writer born and raised in Harlem, experiences extreme emotions during his stay in a 'tiny Swiss village'(127). James Baldwin?s feelings of inadequacy and insecurities of being an American black man bring out a rage in him toward the white man's power over him. His deep feelings of outrage, bringing back hurtful memories as a black child in America, are revived as he is forced to endure the unintentional cruelties of the villagers. Baldwin asserts that even the most simple-minded white man has more control and history over Baldwin than Baldwin's desire to reclaim his culture. As his outrage mounts, Baldwin admits that the black man intends to make the white man stop acknowledging him as an 'exotic rarity and recognize him as a human being'(131). Baldwin further reveals he is tired of getting looked at with curiosity and deception; he desperately yearns to be accepted as an American man. Furthermore, Baldwin's anguish at the loss of the American Negro slave's history, as it was taken away from them, enrages and saddens him, yet he doesn't truly blame the white man because the white Americans were only following in the footsteps of the Europeans of the past. In this context, from Baldwin's perspective, Europeans experienced no dilemma of cons cience towards the black man, and he just 'did not exist for Europe'(132). Americans, on the other hand, faced a moral conflict to recognize the black man?s identity without poisoning their own (133). Despite his rage and aggressive support of the black man?s struggle in America, he strove for humane treatment in a racist society. Baldwin sought to bring about acceptance and a better future for black Americans and having feelings of inadequacy and insecurity determined him to bring change to America: ?This world is white no longer, and it will never be white again?(135). This statement is not meant to be taken at face value.

According to Paul Cezanne Essay

Paul Cezanne’s paintings are regarded today as being of considerable importance to the development of modern art. Cezanne’s use of colour as tone and his heavy interest in the formal elements of composition paved the way for artists following on from him to question what they were perceiving and their ability to visually represent what they saw through painting. This essay will be examining the influence that Paul Cezanne had on Cubism and how this elevated the picture into an autonomous object rather than a mere representation of nature. The French Post-Impressionist painter Paul Cezanne, although heavily ignored by the mainstream art sphere centered within Paris, had a considerable impact on Cubism as he laid out the fundamental ideas from which the cubists would create their works. One of the main key points that was central to the cubists was faceting, this reduction of form within the external world and transforming them to geometrical facets, ‘Nature should be treated as cylinders, spheres and cones.’. See more: Examples of satire in adventures of huckfinn essay Cezanne’s manner of depicting reality with inaccuracy and multiple viewpoints divorced itself from the laws of perceptive construction and allowed him to interpret what he saw in a subjective way, he wasn’t interested in merely imitating what he saw onto the canvas but rather to re-think the conventions which restrict traditional painting. A clear departure from traditional painting as a representational phenomenon has taken place, it’s no longer mimetic of nature or reality but rather holds its own reality within itself and painting and art in general is made autonomous through Cezanne’s unique approach. Where other painters saw transient effects of light Cezanne had the ability to perceive geometric structures and thus from his search for underlying structure of composition came Cubism and then Abstraction. In the impressionist movement, and other predecessors to cubism and post-impressionism, artists weren’t concerned in dealing with matters of inner meaning within their paintings, they were simply responding to what they saw which in turn gave little for the viewer to think about. It is here that we see Cezanne’s more conceptual manner of depicting reality by breaking things into obscure shapes and abstracted form, he projects his inner emotion and consciousness onto the canvas to represent a state of mind, ‘The painter’s idea cannot be conceived apart from the means he uses, for it is meaningful only so far as it is embodied in those means, and the deeper his idea the more complete they must be. I am unable to distinguish between my feelings for life and my way of transposing it. A painting must carry all its meanings within itself, and impose it on the viewer before he identifies the subject matter.’. This parallel of representing nature and self by use of facets and high-keyed colour (colour as a subjective device) is something that’s echoed throughout the works of the cubists, specifically the initial stages of this movement. It seems from this point onwards the painting has become a picture object, it’s removed from the shallow confines of our perception and begins to contribute to reality by adding other aspects and elements, ‘ He was, in fact, the first Post-Impressionist to make, as did the Pointillists and eventually the cubists, as structural analysis of nature, producing an art of the mind concerned with emotional apprehension of formal qualities in mountains, women or onions and totally unconcerned with the evanescent effects of light, shade and atmosphere.’ . This manner of painting had seeped into the consciousness of Pablo Picasso and George Braque, although they had obviously added their own elements to form their own individual styles Ceza nne had laid out the basis from which the cubists could build upon. Although Cubism can be categorised into three key phases: Analytical, Hermetic and Synthetic, it is all somewhat representational of reality. Pushed and stretched to its very limits reality has been distorted and forms are no longer instantly apparent, the viewer must project apart of themselves in order to make sense of what is happening within the painting. From the painters view the cubists applied an aspect of analysis in their paintings by which forms became increasingly fragmented, this way of depicting objects, landscapes and people was similar to Cezanne’s work in the way he represented nature. He was looking beyond the mere surface and impressions which met his gaze and focussed on the inner structures that made up things around him, this of course implies careful consideration and a process of analysis. This is something which resonated with Picasso’s work and contributed to his painting Demoiselles d’ Avingon 1908, ‘He took his cue in this partly from Cezanne’s efforts to fashion a way of painting that would lay bare – or at least open up for exploration by the viewer – the complex process of pictorial representation’. Upon looking at the works of Picasso’s Demoiselles d’ Avingon 1908 and even George Braque’s Houses at L’Estaque 1908, we can indicate a clear influence that is derived from Cezanne’s style of this alignment of nature and the artist. In Braque’s painting we note a visible colour palette which has been simplified and the use of structure that has been used in order to re-create nature, all of which is comparable to Cezanne’s painting Mont Sainte-Victoire 1902-04. However, Braque has accentuated certain elements resulting in a more systematic , clean and simplified style, ‘Yet unlike Cezanne, Braque pushes the juxtaposition of different perspectives to the point of contradiction, and underscores it with a quite arbitrary distribution of light and shade; rooflines fail to meet walls, spaces and solids are elided, buildings are stacked up against one another like playing cards, and in the absence of a horizon the landscape is compress ed into the space of a low relief.’. (FOOTNOTE: Movements in Modern Art Cubism, David Cottington, pg. 22)We notice a clear direction towards further abstraction in terms of form and colour and as cubism progresses these abstractions are further emphasised until reality is barely visible and only signals and indications of things are implied to the viewer. In addition, Picasso’s painting held similar elements of Cezanne’s style and although his painting Demoiselles d’ Avingnon is regarded as more of a proto-cubist artwork in a way it planted the seeds of his style which would later blossom and carry the cubist movement. He is still referencing Cezanne in terms of geometric shapes, limited colours, the fusions between negative and positive shapes and also the arbitrary use of light as he sees fit. Moving away from Demoiselles d’ Avingnon and focussing on Cottage and Trees (La Rue-des-Bois) 1908, again we see a direct correlation in terms of style to Cezanne’s and even Braque’s painting Houses at L’Estaque, in terms of subject matter it’s quite similar; fragmented shapes are used to signify a landscape with houses and contorted trees, however this use of shapes is further abstracted from that of Cezanne’s and Braque’s and again we see the use of colour being completely redundant in terms of imitating the world. The painting has become a complex of invented forms, singular viewpoints, accurate proportions and visual representation are no longer necessary, ‘Picasso independently emphasises each elemnt – volume, line, plane, light and shade – even at the expense of compositional unity. The effect is to maximise the dynamic effect of the painting.’. The emancipation of pictorial elements of line and colour elevated the painting to the picture object, as these elements no longer needed to be used objectively to depict the reality in which we are all submitted to, rather the artists can form the basis of a painting from reality and meet it halfway through his use of representing the internal reality which he is mentally experiencing. Cezanne used a process of analysis and reflection in creating his work which then influenced the cubists to constitute their own individual reality and project it onto the canvas. The result being fragmented geometric shapes which accentuate certain elements of figures, subjective and limited use of colour and an overall less naturalistic representation of what is directly being observed, this in turn helped painters like the cubists to re think what they were painting and also to think of what painting in general could be.

Civilization and Medicinal Uses

1. How is the discovery of beer linked to the emergence of the first civilization?A: The discovery of beer is linked to the emergence of the first civilizations because, a change happened around 12,000 years ago when the nomads deserted there migratory ways, settled down and took up farming. When they began to farm and collect grains that’s when beer was discovered. Therefore beer is linked to the first civilizations emergence by the sudden adoption of farming.2. How is the production of beer an example of plant domestication?A: For something to be domesticated it has to be grown or breed for a specific purpose. Well the plants/grains used to make beer were given just that, a purpose to be grown.3. What sources does Standage use to gather his information on beer?A: Ballinger, Clint. â€Å"Beer Production in the Ancient Near East.† Unpublished paper,   personal communication.Baron, Stanley. Brewed in America: A History of Beer and Ale in the United States.  Boston: L ittle, Brown, 19624. What were some of the uses for beer?A: Beer was as a currency but it was also used medicinally. The workers that built the pyramids were paid in beer and bread, the standard amount each worker got was three or four loafs of bread and two jugs (contains eight liters). Tablets from the Egyptian records that date back to 2100 BCE contains list that different medicinal uses for beer.â€Å"The Ebers Papyrus† yet another medical text for the uses of beer that dates around 1550 BCE , contains many recipes for the medicinal uses of beer but combined with other  ingredients . Half an onion mixed with beer was said to cure constipation.5. How did beer civilize man according to Standage?A: â€Å"Beer permeated the lives of Egyptians and Mesopotamians from the cradle to the grave. Their enthusiasm for it was almost inevitable because of the emergence of complex societies, the need to keep written records, and the popularity of beer all from surplus of grain.† (Standage, 39)What he is saying here is that no one could over look or avoid there excitement for the beer because it brought out more civilized societies, made them keep up with written records to pass down the process to make the beer, and that beer became so popular and so available because of the numerous counts of grains that they had access to know that they had taken up farming and gathering.6. Explain the relationship between beer writing, commerce, and health?A: Beer and writings relationship is that in order for the process of making beer to not only be remembered but also passed down generations, the Egyptians and the Mesopotamians had to make clay tablets. These had the processes, medicinal uses, and ingredients written on their faces. Beer and commerce’s relationship was created when both civilizations used beer as a currency.They used this beverage as means of payment for the builders of the Great Pyramids. On that note this is why the builders on the third Gi za pyramid were known as the â€Å"Drunkards of Menkaure†. Lastly, Beer and healthy relationships come from both civilizations use of this beverage for medicinal purposes. They used ingredients mixed with beer to cure a wide variety of illnesses or discomforts.WINE1. How did the use of wine differ from the use of that in ancient Greece and Rome?A: Wine was seen as a symbol of wealth, he chose not the usual Mesopotamian culture beverage but the elegant wine. Carved stone shows him drinking wine from a bowl not beer. Beer was not used that much for gathering and fest  although it was provided, guest would choose the new drink over there native beer. It was regarded as an exotic drink and it high price and sacristy made it worthy of the gods themselves.2. What did drinking wine symbolize in ancient Greece and Rome?A: Drinking wine in ancient Rome and Greece was an emblem of power, prosperity, and privilege.3. How was wine consumed? Is this similar to or different from beer con sumption in Mesopotamia/Egypt?A: The Pharaohs tasted the wine and made their own vineyards near the deltas of the Nile, for this was a place with rich soil. They made it to where the production outside of their own vineyards was limited. However in Mesopotamia the elegant yet powerful wine was restricted only to that of the wealthy because of the lands incapability to support larger surpluses of the wine.4. How did the use of wine differ in the

Cultural Ethnography, Schein’s Model Essay

Introduction: Cingular Wireless is the largest wireless company in the United States. The company boasts the largest voice and data network and over 58 million customers. In 2004, Cingular generated over $32 billion in revenue. Cingular is owned by AT&T Inc. and Bell South. Cingular‟s vision is, â€Å"To be the most highly regarded wireless company in the world, with a driving focus around best-in-class sales and service† (Cingular, 2006, p.1). The corporation values customers, integrity, performance, teamwork, and its employees The goal of this study was to reveal the organizational culture of Cingular Wireless at a retail sales location. To help determine the culture, this study focused on what is the nature of work for Cingular Wireless sales consultants, and how do employees identify themselves within the corporation according to Edgar Schein‟s models of organizational culture. To guide this study, several aspects of employment at Cingular were studied includin g employee daily routines, flexibility, expectations, and the relationships between managers, sales representatives, and customers. To analyze Cingular, this study will be utilizing Edgar Schein‟s model of organizational cultures. â€Å"Edgar Schein is a management scholar and consultant interested in the role of leaders in the development and maintenance of organizational culture† (Miller, 2006, p. 105). Schein (1992) believes that culture can be studied in levels, which are the degrees to which the culture is visible to observers. His three levels include artifacts, espoused values, and basic underlying assumptions. According to Miller, artifacts are the most obvious in Schein‟s model which consist of the architecture, furniture, technology, dress, written documents, art, forms of address, communication during meets and decision-making styles. (Miller, 2006, p. 107108). â€Å"The problem with artifacts is that they are palpable but hard to decipher accurately. We know how we react to them, but that is not a reliable indicator of how members of the organization react† (Schein, 1990, p. 111-112). The second level of culture Schein recognizes is that of espoused values. Schein‟s espoused values are, â€Å"the articulated, publicly announced principles and values that the group claims to be trying to achieve† (Schein, 1992, p. 9). Schein (1990) believes that the values, ideologies, and norms can be found using interviews, questionnaires, and surveys. Schein believes that, â€Å"Open-ended interviews can be very useful in getting at this level of how people feel and think† (Schein, 1990, p. 112). The third and most difficult level of culture for observers to witness is that of basic assumptions. Miller (2006) believes that members of the group can rarely articulate these basic values since they are a natural part of their daily lives, making the basic assumptions even more difficult for observers to see. From Schein‟s models and definitions it is apparent that the Schein views, â€Å"culture as a complex pattern of assumptions, values, behaviors, and artifacts† (Miller, 2006, p. 111). Schein (1990) believes that through intense observation and the involvement of motivated employees usually unconscious assumptions and perceptions can be discovered about the organizations culture. Schein states, â€Å"Working with motivated insiders is essential because only they can bring to the surface their own underlying assumptions and articulate how they basically perceive the world around them† (Schein, 1990, p. 112). In addition to these three basic levels this study will also utilize the ten major categories Schein uses to help define an organization‟s culture. These will include (Schein, 1992).: 1. Observed behavioral regularities when people interact 2. Group Norms 3. Espoused values 4. Formal philosophy 5. Rules of the Game 6. Climate 7. Embedded skills 8. Habits of thinking, mental models, and/or linguistic paradigms 9. Shared meanings 10. â€Å"Root metaphors† or integrating symbols Although Schein‟s models are widely applied and studied, areas of disagreement with his methods include how much time is actually required to reveal the levels of an organization‟s culture. How many participants are needed to properly assess the culture, and if bias affects the review of an organization also can all hinder the accuracy of a study based off of Schein‟s model. To conduct this study eight of Schein‟s ten major categories were incorporated. The first, observed behavioral regularities are the languages, rituals, traditions and routines of employees. The second category is espoused values which are publicly known goals of the group. The third category is the formal philosophy which is company policies and interactions with customers. The fourth category will be the rules of the game which are the things new employees must learn to fit in. Embedded skills and shared meanings will be the fifth and sixth categories incorporated into this study. These are the necessary skills to do the job and how the employees have similar meanings for certain things. Finally, the basic assumptions will be the eighth category incorporated into the study of the organizational culture of Cingular Wireless. (Schein, 1992) Methodology: To discover how Cingular fits into Schein‟s categories of organizational culture a method f participant observation will be used including observing daily routines, employee interactions amongst themselves and customers, and participating as a member of the Cingular staff at a retail store location. This ethnographic approach is influenced by an article Esteban, Hirt and McGuire. The article incorporated Schein‟s model in a study on â€Å"The Work Life of Student Service Professionals at Rural Community Colleges. The article was helpful in the organization of this study as well as an example of different methods of conducting the study itself. (Esteban, 2003). Applying Schein‟s Model To Cingular Wireless-7 For this study, information from Cingular Wireless was collected including orientation information, employee handbooks, daily forms and corporate publications. In addition, the company owned retail store at Marley Station Mall in Glen Burnie, Maryland was extensively observed and studied. The store has a manager, three full-time employees, and one part-time employee that were interviewed as well. These methods are being used because they will provide a window for gaining insight into the culture of Cingular Wireless. These methods will reveal the way the organizational culture is shaped by the management, employees, and corporate headquarters. The methods are better than others in this situation because it will allow the study to examine a variety of aspects rather than only one or two. Participants: Jason Celani, 34, has been the manager of the Marley Station store since its opening in the fall of 2005. Celani was a former AT&T employee and has been in the wireless industry for about 8 years. His job consists of scheduling staff, ordering products, conducting inventory checks, reporting to a regional manager, conducting weekly meetings, and attending training sessions. Jason loves technology and enjoys reading about new products in his spare time. (J. Celani, Personal Communication, 11/06/2006). Tracy Corcoran, 39, is a full-time employee that has been in the wireless industry for 6 years. Previously Corcoran was also an AT&T employee. He is the senior sales representative at the Marley Station store and is not very interested in new technology. Tracy is hoping to get out of the wireless industry within the next few years. (T. Corcoran, Personal Communcation, 10/28/2006). Is a part-time college student that has been working at the Marley Station store since March 2006. She enjoys having the newest phone on the market and loves being able to get her email wherever she goes. She has no previous experience in the wireless industry. (L. Andes-Miller, Personal Communication, 10/27/2006). Chuck Payne is the newest full-time employee at the store. Payne is 20 years old and a former radio broadcaster for a gospel station in the Washington D.C. area. Payne has never worked in the wireless industry but also enjoys getting new phones when they come out. Payne began his employment in late July of 2006. (C. Payne, Personal Communication, 11/01/2006). Alejandro Quant is the only part-time employee observed and interviewed at the Marley Station store. Quant, 21, is a full-time student at the University of Maryland. Quant is valuable to the location because of previous job experience with T Mobile, another wireless provider and also because he is bilingual. He speaks both Spanish and English. (A. Quant, Personal Communication, 11/05/2006). The participants were all observed and interviewed and the way in which they interacted was noted. They were asked about likes and dislikes, daily operations, and if they saw a future within the company. This study was conducted at the Cingular Wireless at Marley Station by a parttime employee that has been with the company since June 2006. As an embedded observer internal access to the organizational culture of the Cingular store was gained. The research for this ethnography also benefited because as an employee the basic workings of the company was easily accessible, the other employees are not uncomfortable interacting with me, and access to internal documents and meetings was possible. The goals of this ethnography are to gain a better understanding of the basic assumptions of the organization. The representatives studied work long hours and they have a good grasp on the organizational culture of Cingular. To bracket avoid bias in this study observations and interviews were conducted off the clock. Time was spent observing the employees out on the sales floor and also via the security cameras located in the office. Also to avoid bias, interviews were conducted outside of the work environment at local restaurants. They were not conducted during or after scheduled shifts. Materials: A variety of materials and sources will be used to support this study. This will include orientation and training documents, internal employee handbooks, external documents, interviews with employees, and on site observations. Analysis of Data: To analyze the data collected in the study of Cingular Wireless at Marley Station Mall this portion will be organized according to eight of Schein‟s categories utilized for this study. They will be supported using internal and external documents, observations, and employee interviews. Artifacts: The Marley Station Cingular store had a plethora of easily observable artifacts. The most apparent was the colors and logo of Cingular all over the store. There was no furniture and a very linear floor pan. In general all of the staff members said that they disliked the floor plan because standing all day got tiring and it was hard to maneuver around the store when it got crowded because it was small and the counter‟s linear design made it impossible to get around each other. The colors of orange and blue were on most signs and popular slogans such as â€Å"raising the bar,† â€Å"roll over minutes,† and â€Å"All over network† were visible all over the store. The floor plan pushed what Cingular refers to as its advantages over other wireless carriers. Cingular shirts and name tags were the next most obvious artifact. All of the employees wear Cingular branded shirts and a name tag on the right hand side. Cingular has a contract with Lands End clothing company to customize what the company refers to as its â€Å"Team Colors.† (See Appendix A) Each employee is allowed $125 for their spring wardrobe and $175 for their fall and winter uniforms. Specific vocabulary and language is very obvious at the store. Celani often sends text-messages to all of his employees at the end of the business day and he refers to them collectively as â€Å"team.† When a representative has customers come in, they are referred to as an â€Å"opportunity,† and extras that can be added onto a calling plan are referred to as a â€Å"bolt-on† (J. Celani, Personal Communication, 11/01/2006). Cingular boasts that it has the largest product variety in the wireless industry. The store itself has a large amount of products on the wall which are referred to as „live.‟ This means that the phones are in working condition and the customers can try them out in the stores. â€Å"At de alers the phones on the walls are dummies, customers can‟t try them out† said Andes-Miller (L. Andes-Miller, Personal Communication, 10/27/2006). There is a large amount of documentation conducted at Cingular Wireless stores. Every morning a form titled â€Å"Non-Negotiable Standards† is filled out by employees. (See Appendix B) This is a check list of standards such as cleanliness, work attire, and merchandise that is to be completed before the store opens. â€Å"The non-negotiables are like getting a cup of coffee in the morning, we all are used to doing them,† said Corcoran (T.Corcoran, Personal Communication, (10/28/2006). Every time a representative makes a sale or adds on to a customers account they are also required to mark it on the â€Å"Daily Sales Record† sheet. (See Appendix C) Observed Behavioral Regularities: Cingular has what they refer to as a six step sales process. (See Appendix D) Each employee is introduced to this on their first day of new-hire training, which is a program that introduces new employees to the Cingular way of selling. According to this process each customer is to be greeted within 10 feet/10 seconds of entering the store. The representatives at the Marley Station store do this without thinking. Also included in this process is building value for the customer, offering solutions, asking for the sale, educating the customer, and thanking the customer. In addition to a set sales process each representative knows to sell what is referred to as â€Å"The Cingular Advantage.† (See Appendix D) All of the representatives are required to have skills on a computer system called Opus. This is the computer system which allows the representatives access, create, and change accounts. â€Å"Opus is much easier to use than past systems,† said veteran employee Corcoran, â€Å"It freezes but we all know how to deal with it. Each of us uses the same system and procedures every day to work on and verify accounts† (T. Corcoran, Personal Communication, 10/28/2006). Every employee realizes that they must attend a meeting every Friday morning before the store opens. At this meeting the floor plan is discussed, scheduling is worked out, and any other issues are brought up. The communication between the employees and the managers is very informal. Everyone has a chance to speak and the staff knows that once all business is completed they will go out to breakfast. It is very obvious that Cingular‟s first level of organizational culture, the artifacts, is prevalent through out the store. Employees have a common vocabulary specific to their work, they have uniforms, a system for selling and a store that advertises what the company is all about, being the best wireless provider. Espoused Values: The team at the Marley Station Cingular store is constantly striving to achieve goals which are advertised by the wireless company. While working with customers the sales representatives know that they need to promote the Cingular Advantages. The Cingular Advantages include the best technology, great value, best products and services, newest handsets, and the most convenient services. (See Appendix D) â€Å"Orientation and training pounds these ideas into our heads from the first day we have our jobs,† said Quant. â€Å"Cingular has high standards that we all must uphold† (A. Quant, Personal Communication, 11/05/2006). To ensure that the representatives uphold the values of the company Cingular sends out mystery shoppers to each store at least once a month to make sure that the representatives are selling in accordance to Cingular policy. The mystery shoppers have a checklist and grade the representatives. (See Appendix E) The company also makes what it values available to the general public. On its website Cingular lists its goals and core values, as well as their definitions and what the values mean to the company. (See Appendix F) Basic Assumptions: The basic assumptions and values of Cingular wireless are hard to observe as both an outsider and as an employee initially. After extensive observation, job experience, and interviews there are some basic assumptions that surface. The first is that the organization values employees that are „hungry.‟ If it is a slow day at the store employees print out flyers and distribute them to local business or make calls to customers asking how their service is going in hopes of adding onto their plans. These behaviors are highly praised by the manager of the store for being innovative and going above and beyond the job description. An example of this is employees distributing flyers outside of their scheduled hours for events such as â€Å"Friends and Family Day,† which gives customers extra discounts for coming in on designated dates. (See Appendix G) Unlike many full-time workers that have benefits the employees of the Marley Station store expect to work nights and weekends. If an employee begins to gripe about working these hours the other employees tend to remind them that that is just the way things are. Finally, all employees seem to understand that they are expendable by the company and do not question quotas, paperwork, or seemingly superfluous procedures that waste time and do not make sense. New employees tend to learn quickly the way are and solutions that have been turned into operational policies. All staff members know to let a customer know that not all phone numbers may be successfully transferred when they upgrade their phone but they will do their best. Very rarely are numbers unable to be transferred but employees know the steps necessary to cover themselves in case rare things actually happen. â€Å"I didn‟t understand half the stuff when I started working here, I thought that Friday morning meetings were stupid, and I did not want to be here at 9am on Saturdays. Now I just how things are, I didn‟t realize that those things were a part of Cingular‟s culture. I didn‟t even know Cingular had a culture, but I guess that makes sense,† said Payne (C. Payne, Personal Communication, 11/01/2006). Conclusion: This study tried to examine the organizational culture of Cingular Wireless in relation to Schein‟s model and his levels of artifacts, espoused values, and basic assumptions. The study would have been improved if there was an employee in the process of leaving the company that an exit interview could have been conducted on. This would have benefited the study because it would probably have uncovered some downfalls of the culture of Cingular. The next step and question in this study would have been to compare the employee experiences at the Marley Station Mall store to employees at a stand-alone store or at a kiosk to see if their experiences were any different. Finally, the implication of this study on later discussions of the corporation is that this study provides a solid base for a person trying to research this wireless provider in the Washington D.C./Baltimore market. References Cingular Wireless. (2006). 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Organizational culture and leadership (2nd ed.) San Francisco, Ca: †¦Ã¢â‚¬ ¦.Josey-Bass Publishers.